2.cuatro.step 3. Sequencing from DNA

Having DNA sequencing, the fresh amplification facts extracted from the fresh new PCR reactions was in fact refined which have new Divide II PCR and you will Serum System (Bioline, London area, UK) and you may sequenced inside an enthusiastic Avant 3100 sequencer (Used Biosystem, Waltham, MA, USA). DNA sequencing is actually performed from the a sequencing business in the School from Veterinarian Medication, Kosice, Slovakia. This new received DNA sequences on the limited bg and you may tpi genetics was in fact compared to brand new reference sequences off Grams. duodenalis in the GenBank databases from the nucleotide BLASTn system . Sequences was in fact arranged from the similarity and aligned with the Clustal Omega tool . Phylograms was indeed created because of the MEGA7 software utilising the restriction-likelihood (ML) means having 1000 bootstrap pseudoreplicates. A great TN93 substitution design that have gamma-marketed price heterogeneity is chose according to research by the restrict chances research away from it is possible to replacing activities with the Modeltest during the Super.

2.5. Statistical Analysis

Statistical analysis of the obtained results was performed using the statistical product StatSoft, Inc. (2007). STATISTICA (software system for data analysis), version 8.0. and StatSoft Statistica 12 and Microsoft Excel (Office 365). The potential risk was calculated using the exchange rate (OR) as a relative chance and the relative risk using the risk rate (RR). Crosstables and Chi-square tests were used to calculate correlations between sex, the children’s residence belonging to a majority or minority population, and age-class. (more…)